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Glossary

Orthogonal method

An orthogonal analytical method relies on a different separation or detection principle from the primary method, such as capillary electrophoresis or ion-exchange alongside reversed-phase HPLC. Using orthogonal methods reduces the risk that impurities co-eluting with the main peak go undetected, giving a more reliable assessment of peptide purity.

Source: americanpharmaceuticalreview.com

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