Glossary

Monoisotopic mass

Monoisotopic mass is the mass of a molecule calculated using the most abundant isotope of each element, such as carbon-12, hydrogen-1, nitrogen-14 and oxygen-16. High-resolution mass spectrometers resolve isotopic peaks, so the monoisotopic peak is used to confirm peptide identity. It is lower than the average mass, especially for larger peptides.

Source: en.wikipedia.org

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