Glossary

Edman degradation

Edman degradation is a method of sequencing peptides by sequentially removing and identifying one amino acid at a time from the N-terminus using phenyl isothiocyanate. Developed by Pehr Edman, it requires a free N-terminus and is practical for up to about 30 residues, with an upper limit of roughly 50 to 60. Mass spectrometry has largely supplanted it.

Source: en.wikipedia.org

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