What is the amino acid sequence of tirzepatide?
Tirzepatide is a research peptide whose amino acid sequence is on the public registry record. The full published sequence is listed below; it includes non-standard residues and side-chain modifications as written in the source record. Its molecular formula is C225H348N48O68. Its molecular weight is 4813.0 g/mol.
Sequence. H-Tyr-Aib-Glu-Gly-Thr-Phe-Thr-Ser-Asp-Tyr-Ser-Ile-Aib-Leu-Asp-Lys-Ile-Ala-Gln-Lys(N6-[(22S)-22,42-dicarboxy-10,19,24-trioxo-3,6,12,15-tetraoxa-9,18,23-triazadotetracontan-1-oyl])-Ala-Phe-Val-Gln-Trp-Leu-Ile-Ala-Gly-Gly-Pro-Ser-Ser-Gly-Ala-Pro-Pro-Pro-Ser-NH2 (Lys side chain as named in WHO Proposed INN List 119, WHO Drug Information Vol. 32 No. 2, 2018, p. 370)
Class. peptide (lipidated).
Formula. C225H348N48O68.
Mass. 4813.0 g/mol.
Analytical note. Identity confirmation by mass spectrometry uses the mass calculated from the formula. Sable's release specification is ≥99% by HPLC with identity confirmed against the expected mass (quality page). Research use only. Not a medicine. Not for human or veterinary use.
Sources
- https://www.ebi.ac.uk/chembl/api/data/molecule/CHEMBL4297839.json
- https://pubchem.ncbi.nlm.nih.gov/compound/166567236
See the Tirzepatide research hub for every Sable page on Tirzepatide.
Tirzepatide: reference facts
7 Tirzepatide values, each linked to its public source; see the Tirzepatide reference page.
| Synonyms | LY3298176 (pubchem.ncbi.nlm.nih.gov) |
|---|---|
| Compound class | peptide (lipidated) (pubchem.ncbi.nlm.nih.gov) |
| CAS Registry Number | 2023788-19-2 (pubchem.ncbi.nlm.nih.gov) |
| Molecular formula | C225H348N48O68 (pubchem.ncbi.nlm.nih.gov) |
| Molecular weight | 4813.0 g/mol (pubchem.ncbi.nlm.nih.gov) |
| PubChem CID | 166567236 (pubchem.ncbi.nlm.nih.gov) |
| Storage (lyophilised) | -20 °C, dry, protected from light (sigmaaldrich.com) |
Sizes and prices at Sable
| Vial | Price | Per mg | UK same-size check (2026-10-16) |
|---|---|---|---|
| 5 mg | £24.93 | £4.99 | lowest other £24.95 across 1 UK sellers |
| 10 mg | £44.98 | £4.50 | no same-size match |
| 15 mg | £38.98 | £2.60 | no same-size match |
| 20 mg | £40.39 | £2.02 | no same-size match |
| 30 mg | £61.98 | £2.07 | no same-size match |
| 40 mg | £86.98 | £2.17 | no same-size match |
| 60 mg | £119.98 | £2.00 | no same-size match |
Multi-vial pricing on the same item: 3+ vials 10% off, 5+ vials 15% off, 10+ vials 20% off. Every lot has a certificate of analysis: CoA library.
Typical analytical method
| HPLC column | C18 reversed-phase (300 A pore, C4/C8 suitable for large/lipidated) |
|---|---|
| Mobile phase | A: water + 0.1% TFA; B: acetonitrile + 0.1% TFA; linear gradient (e.g. 5-65% B over 20-30 min) |
| Detection | 214-220 nm (peptide bond) and 280 nm (Trp/Tyr present) |
General literature method. The lot CoA records the method actually used. How to read an HPLC chromatogram.
Related questions
Lyophilised storage on file: Tirzepatide
From the Sable storage reference (lyophilised material only; temperature, light, moisture). Literature basis is linked per row. See peptide storage.
| Compound | Lyophilised storage | Light / notes |
|---|---|---|
| Tirzepatide | -20 C long term (-80 C acceptable), sealed and desiccated; 2-8 C for weeks | protect from light; contains Met/Cys/Trp: oxidation-prone; minimise air/headspace exposure (source) |
Typical analytical method: Tirzepatide
Reference HPLC and MS conditions from the Sable analytical-methods file; a lot's CoA states the method actually used. See how to read a CoA.
| Compound | HPLC column / detection | MS / expected ion |
|---|---|---|
| Tirzepatide | C18 reversed-phase (300 A pore, C4/C8 suitable for large/lipidated); 214-220 nm (peptide bond) and 280 nm (Trp/Tyr present) | ESI-MS (positive mode); LC-MS for identity; [M+3H]3+ ~1605.3; [M+4H]4+ ~1204.3; [M+5H]5+ ~963.6; [M+6H]6+ ~803.2 |
Research use only. Not a medicine. Not for human or veterinary use.