Glossary

Ion-pairing agent

An ion-pairing agent is a mobile-phase additive that forms neutral or less polar ion pairs with charged analytes, modifying their retention in reversed-phase chromatography. Trifluoroacetic acid is the classic ion-pairing agent for peptides, improving peak shape. Formic acid is weaker but more compatible with mass spectrometry.

Source: pubmed.ncbi.nlm.nih.gov

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