Glossary

Ellman's reagent

Ellman's reagent, 5,5'-dithiobis(2-nitrobenzoic acid) or DTNB, reacts with free thiol groups to release a yellow product that absorbs at 412 nm. It is used to quantify free cysteine thiols in peptides, for example to confirm complete disulfide formation or the availability of thiols for conjugation.

Source: en.wikipedia.org

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