Glossary

Biotinylation

Biotinylation is the covalent attachment of biotin to a molecule such as a peptide. Biotin binds streptavidin and avidin with extremely high affinity, so biotinylated peptides are used for immobilisation, pull-down experiments and detection in assays such as ELISA. Spacers are often included to reduce steric hindrance.

Source: en.wikipedia.org

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