---
title: "Reconstitution concentration calculator (mg/mL) | Sable Peptides"
description: "Lab calculator: enter the peptide mass in the vial (mg) and the solvent volume added (mL) to get the solution concentration in mg/mL. Research use only."
canonical: https://www.sablepeptides.co.uk/guides/reconstitution-concentration-calculator
publisher: Sable Peptides Limited (UK company 17488610)
note: Research use only. Not for human or veterinary use.
---

# Reconstitution concentration calculator

Concentration (mg/mL) = peptide mass (mg) ÷ solvent volume (mL). Enter the mass stated for the vial and the volume of solvent added.

Peptide mass in vial (mg)Solvent volume added (mL)

Concentration: **5 mg/mL**

## Worked examples

| Mass | Volume | Concentration |
| --- | --- | --- |
| 5 mg | 1 mL | 5 mg/mL |
| 5 mg | 2 mL | 2.5 mg/mL |
| 10 mg | 1 mL | 10 mg/mL |
| 10 mg | 2 mL | 5 mg/mL |
| 10 mg | 5 mL | 2 mg/mL |
| 50 mg | 5 mL | 10 mg/mL |

## Notes for the lab

-   The result uses the stated vial mass. Net peptide content is usually lower than gross weight because lyophilised peptides contain water and counter-ions; if the lot CoA gives a measured content, use that figure for a more exact concentration.
-   The volume added is treated as the final volume. For small volumes the dissolved solid adds slightly to the total.
-   Solvent choice depends on the peptide's solubility: see [storage and solubility](/learn/peptide-storage-and-solubility).
-   Solution stability: see [oxidation-prone residues](/guides/oxidation-prone-residues-in-peptide-solutions) and [peptide aggregation in assay solutions](/guides/peptide-aggregation-in-assay-solutions).

This calculator gives a solution concentration only. It does not give, and must not be used to work out, any administration amount. Research use only. Not for human or veterinary use.