---
title: "UV detector: research glossary | Sable Peptides"
description: "A UV detector measures the absorbance of ultraviolet light by compounds eluting from an HPLC column. Peptide bonds absorb strongly near 214 nm, and aromati"
canonical: https://www.sablepeptides.co.uk/glossary/uv-detector
publisher: Sable Peptides Limited (UK company 17488610)
note: Research use only. Not for human or veterinary use.
---

Glossary

# UV detector

A UV detector measures the absorbance of ultraviolet light by compounds eluting from an HPLC column. Peptide bonds absorb strongly near 214 nm, and aromatic residues such as tryptophan and tyrosine absorb around 280 nm. Detection at 214 to 220 nm is used for peptide purity because all peptides respond.

Source: [en.wikipedia.org](https://en.wikipedia.org/wiki/Ultraviolet%E2%80%93visible_spectroscopy)

## Related terms

-   [Peptide](/glossary/peptide)
-   [Peptide purity](/glossary/peptide-purity)
-   [Absorbance](/glossary/absorbance)
-   [Tryptophan](/glossary/tryptophan)
-   [Tyrosine](/glossary/tyrosine)

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