---
title: "Orthogonal method: research glossary | Sable Peptides"
description: "An orthogonal analytical method relies on a different separation or detection principle from the primary method, such as capillary electrophoresis or ion-e"
canonical: https://www.sablepeptides.co.uk/glossary/orthogonal-method
publisher: Sable Peptides Limited (UK company 17488610)
note: Research use only. Not for human or veterinary use.
---

Glossary

# Orthogonal method

An orthogonal analytical method relies on a different separation or detection principle from the primary method, such as capillary electrophoresis or ion-exchange alongside reversed-phase HPLC. Using orthogonal methods reduces the risk that impurities co-eluting with the main peak go undetected, giving a more reliable assessment of peptide purity.

Source: [americanpharmaceuticalreview.com](https://www.americanpharmaceuticalreview.com/Featured-Articles/182871-The-Role-of-Complimentary-Methods-in-Analytical-Quality-Control/)

## Related terms

-   [Peptide](/glossary/peptide)
-   [Peptide purity](/glossary/peptide-purity)
-   [Capillary electrophoresis](/glossary/capillary-electrophoresis)

[Back to glossary A–Z](/glossary/terms)

All Sable Peptides products are supplied for laboratory research use only. Not for consumption.