---
title: "N-methylation: research glossary | Sable Peptides"
description: "N-methylation is the replacement of a backbone amide hydrogen with a methyl group. In peptides it removes a hydrogen-bond donor, can increase membrane perm"
canonical: https://www.sablepeptides.co.uk/glossary/n-methylation
publisher: Sable Peptides Limited (UK company 17488610)
note: Research use only. Not for human or veterinary use.
---

Glossary

# N-methylation

N-methylation is the replacement of a backbone amide hydrogen with a methyl group. In peptides it removes a hydrogen-bond donor, can increase membrane permeability and resistance to proteases, and restricts conformation. N-methylated residues are harder to couple in synthesis and often require strong coupling reagents such as HATU.

Source: [en.wikipedia.org](https://en.wikipedia.org/wiki/Methylation)

[Back to glossary A–Z](/glossary/terms)

All Sable Peptides products are supplied for laboratory research use only. Not for consumption.