---
title: "Mobile phase: research glossary | Sable Peptides"
description: "The mobile phase is the liquid or gas that carries the sample through a chromatography column. In reversed-phase peptide HPLC it usually consists of water"
canonical: https://www.sablepeptides.co.uk/glossary/mobile-phase
publisher: Sable Peptides Limited (UK company 17488610)
note: Research use only. Not for human or veterinary use.
---

Glossary

# Mobile phase

The mobile phase is the liquid or gas that carries the sample through a chromatography column. In reversed-phase peptide HPLC it usually consists of water (solvent A) and acetonitrile (solvent B), each containing about 0.1% trifluoroacetic acid or formic acid. Its composition controls retention and selectivity.

Source: [en.wikipedia.org](https://en.wikipedia.org/wiki/Mobile_phase)

## Related terms

-   [Peptide](/glossary/peptide)
-   [Trifluoroacetic acid](/glossary/trifluoroacetic-acid)
-   [Acetonitrile](/glossary/acetonitrile)
-   [Formic acid](/glossary/formic-acid)

[Back to glossary A–Z](/glossary/terms)

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