---
title: "Isocratic elution: research glossary | Sable Peptides"
description: "Isocratic elution is an HPLC mode in which the mobile phase composition remains constant throughout the run. It is simple and reproducible but less suitabl"
canonical: https://www.sablepeptides.co.uk/glossary/isocratic-elution
publisher: Sable Peptides Limited (UK company 17488610)
note: Research use only. Not for human or veterinary use.
---

Glossary

# Isocratic elution

Isocratic elution is an HPLC mode in which the mobile phase composition remains constant throughout the run. It is simple and reproducible but less suitable for peptide mixtures with widely differing retention, where late-eluting components produce broad peaks. Gradient elution is therefore more common for peptides.

Source: [en.wikipedia.org](https://en.wikipedia.org/wiki/High-performance_liquid_chromatography#Isocratic_and_gradient_elution)

## Related terms

-   [Peptide](/glossary/peptide)
-   [Mobile phase](/glossary/mobile-phase)
-   [Gradient elution](/glossary/gradient-elution)

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