---
title: "Ion-pairing agent: research glossary | Sable Peptides"
description: "An ion-pairing agent is a mobile-phase additive that forms neutral or less polar ion pairs with charged analytes, modifying their retention in reversed-pha"
canonical: https://www.sablepeptides.co.uk/glossary/ion-pairing-agent
publisher: Sable Peptides Limited (UK company 17488610)
note: Research use only. Not for human or veterinary use.
---

Glossary

# Ion-pairing agent

An ion-pairing agent is a mobile-phase additive that forms neutral or less polar ion pairs with charged analytes, modifying their retention in reversed-phase chromatography. Trifluoroacetic acid is the classic ion-pairing agent for peptides, improving peak shape. Formic acid is weaker but more compatible with mass spectrometry.

Source: [pubmed.ncbi.nlm.nih.gov](https://pubmed.ncbi.nlm.nih.gov/16213445/)

## Related terms

-   [Trifluoroacetic acid](/glossary/trifluoroacetic-acid)
-   [Reversed-phase chromatography](/glossary/reversed-phase-chromatography)
-   [Formic acid](/glossary/formic-acid)
-   [Mass spectrometry](/glossary/mass-spectrometry)

[Back to glossary A–Z](/glossary/terms)

All Sable Peptides products are supplied for laboratory research use only. Not for consumption.