---
title: "High-performance liquid chromatography: research glossary | Sable Peptides"
description: "High-performance liquid chromatography (HPLC) is a technique that separates the components of a liquid sample by pumping it at high pressure through a colu"
canonical: https://www.sablepeptides.co.uk/glossary/high-performance-liquid-chromatography
publisher: Sable Peptides Limited (UK company 17488610)
note: Research use only. Not for human or veterinary use.
---

Glossary

# High-performance liquid chromatography

High-performance liquid chromatography (HPLC) is a technique that separates the components of a liquid sample by pumping it at high pressure through a column packed with fine particles. Components interact differently with the stationary phase and elute at different times. HPLC with UV detection is the standard method for assessing synthetic peptide purity.

Source: [en.wikipedia.org](https://en.wikipedia.org/wiki/High-performance_liquid_chromatography)

## Related terms

-   [Peptide](/glossary/peptide)
-   [Peptide purity](/glossary/peptide-purity)
-   [Stationary phase](/glossary/stationary-phase)

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