---
title: "HEPES: research glossary | Sable Peptides"
description: "HEPES is a zwitterionic Good's buffer with a pKa of about 7.5 at 25 °C, commonly used in cell culture and biochemical assays to maintain physiological pH o"
canonical: https://www.sablepeptides.co.uk/glossary/hepes
publisher: Sable Peptides Limited (UK company 17488610)
note: Research use only. Not for human or veterinary use.
---

Glossary

# HEPES

HEPES is a zwitterionic Good's buffer with a pKa of about 7.5 at 25 °C, commonly used in cell culture and biochemical assays to maintain physiological pH outside a carbon dioxide incubator. It has low metal binding, but can generate hydrogen peroxide when exposed to light in the presence of riboflavin.

Source: [en.wikipedia.org](https://en.wikipedia.org/wiki/HEPES)

## Related terms

-   [Buffer](/glossary/buffer)
-   [Cell culture](/glossary/cell-culture)

[Back to glossary A–Z](/glossary/terms)

All Sable Peptides products are supplied for laboratory research use only. Not for consumption.