---
title: "Gradient elution: research glossary | Sable Peptides"
description: "Gradient elution is an HPLC mode in which the mobile phase composition changes during the run, typically increasing the organic solvent proportion over tim"
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---

Glossary

# Gradient elution

Gradient elution is an HPLC mode in which the mobile phase composition changes during the run, typically increasing the organic solvent proportion over time. It allows peptides with a wide range of hydrophobicities to elute as sharp peaks within a reasonable run time and is standard for peptide purity analysis.

Source: [en.wikipedia.org](https://en.wikipedia.org/wiki/High-performance_liquid_chromatography#Isocratic_and_gradient_elution)

## Related terms

-   [Peptide](/glossary/peptide)
-   [Peptide purity](/glossary/peptide-purity)
-   [Mobile phase](/glossary/mobile-phase)

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