---
title: "Freeze-thaw cycle: research glossary | Sable Peptides"
description: "A freeze-thaw cycle is a single round of freezing a sample and then thawing it. Repeated cycles can damage peptides and proteins through ice-crystal format"
canonical: https://www.sablepeptides.co.uk/glossary/freeze-thaw-cycle
publisher: Sable Peptides Limited (UK company 17488610)
note: Research use only. Not for human or veterinary use.
---

Glossary

# Freeze-thaw cycle

A freeze-thaw cycle is a single round of freezing a sample and then thawing it. Repeated cycles can damage peptides and proteins through ice-crystal formation, local concentration of solutes, pH shifts and air-water interface exposure, promoting aggregation and degradation. Aliquoting stock solutions avoids the need to thaw the whole sample repeatedly.

Source: [en.wikipedia.org](https://en.wikipedia.org/wiki/Freeze-thaw)

[Back to glossary A–Z](/glossary/terms)

All Sable Peptides products are supplied for laboratory research use only. Not for consumption.