---
title: "Edman degradation: research glossary | Sable Peptides"
description: "Edman degradation is a method of sequencing peptides by sequentially removing and identifying one amino acid at a time from the N-terminus using phenyl iso"
canonical: https://www.sablepeptides.co.uk/glossary/edman-degradation
publisher: Sable Peptides Limited (UK company 17488610)
note: Research use only. Not for human or veterinary use.
---

Glossary

# Edman degradation

Edman degradation is a method of sequencing peptides by sequentially removing and identifying one amino acid at a time from the N-terminus using phenyl isothiocyanate. Developed by Pehr Edman, it requires a free N-terminus and is practical for up to about 30 residues, with an upper limit of roughly 50 to 60. Mass spectrometry has largely supplanted it.

Source: [en.wikipedia.org](https://en.wikipedia.org/wiki/Edman_degradation)

## Related terms

-   [Amino acid](/glossary/amino-acid)
-   [N-terminus](/glossary/n-terminus)
-   [Mass spectrometry](/glossary/mass-spectrometry)

[Back to glossary A–Z](/glossary/terms)

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