---
title: "C18 column: research glossary | Sable Peptides"
description: "A C18 column is an HPLC column packed with silica particles bonded with octadecyl (18-carbon) chains, providing a strongly hydrophobic stationary phase. It"
canonical: https://www.sablepeptides.co.uk/glossary/c18-column
publisher: Sable Peptides Limited (UK company 17488610)
note: Research use only. Not for human or veterinary use.
---

Glossary

# C18 column

A C18 column is an HPLC column packed with silica particles bonded with octadecyl (18-carbon) chains, providing a strongly hydrophobic stationary phase. It is the most widely used column type for reversed-phase peptide analysis. Wide-pore (around 300 Å) versions are preferred for larger peptides and proteins to allow access to the pores.

Source: [en.wikipedia.org](https://en.wikipedia.org/wiki/Reversed-phase_chromatography)

## Related terms

-   [Peptide](/glossary/peptide)
-   [Stationary phase](/glossary/stationary-phase)

[Back to glossary A–Z](/glossary/terms)

All Sable Peptides products are supplied for laboratory research use only. Not for consumption.