---
title: "Biotinylation: research glossary | Sable Peptides"
description: "Biotinylation is the covalent attachment of biotin to a molecule such as a peptide. Biotin binds streptavidin and avidin with extremely high affinity, so b"
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publisher: Sable Peptides Limited (UK company 17488610)
note: Research use only. Not for human or veterinary use.
---

Glossary

# Biotinylation

Biotinylation is the covalent attachment of biotin to a molecule such as a peptide. Biotin binds streptavidin and avidin with extremely high affinity, so biotinylated peptides are used for immobilisation, pull-down experiments and detection in assays such as ELISA. Spacers are often included to reduce steric hindrance.

Source: [en.wikipedia.org](https://en.wikipedia.org/wiki/Biotinylation)

## Related terms

-   [Peptide](/glossary/peptide)
-   [ELISA](/glossary/elisa)

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